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pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)
(Plasmid #194190)

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Item Catalog # Description Quantity Price (USD)
Plasmid 194190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMANTIS promoter (1kb)
  • Alt name
    MANTIS promoter (1kb)
  • Alt name
    AK125871 promoter (1kb)
  • Species
    H. sapiens (human)
  • Mutation
    5 mutated KLF binding sites (positions 449,548,732,899,884)
  • Entrez Gene
    SMANTIS (a.k.a. ANXA4-AS, n342419)
  • Promoter SMANTIS promoter (1kb), mutated

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All) was a gift from Ralf Brandes & Matthias Leisegang (Addgene plasmid # 194190 ; http://n2t.net/addgene:194190 ; RRID:Addgene_194190)

  • For your References section:

    Pleiotropic effects of laminar flow and statins depend on the Kruppel-like factor-induced lncRNA MANTIS. Leisegang MS, Bibli SI, Gunther S, Pfluger-Muller B, Oo JA, Hoper C, Seredinski S, Yekelchyk M, Schmitz-Rixen T, Schurmann C, Hu J, Looso M, Sigala F, Boon RA, Fleming I, Brandes RP. Eur Heart J. 2019 Aug 7;40(30):2523-2533. doi: 10.1093/eurheartj/ehz393. 10.1093/eurheartj/ehz393 PubMed 31222221
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