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Addgene

pCMV6-ZNF354C_mutant_D17A-V18A
(Plasmid #194184)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194184 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZNF354C
  • Alt name
    KID3
  • Species
    H. sapiens (human)
  • Mutation
    KRAB domain mutant D17A/V18A
  • Entrez Gene
    ZNF354C (a.k.a. KID3)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc-DDK (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6-ZNF354C_mutant_D17A-V18A was a gift from Ralf Brandes & Matthias Leisegang (Addgene plasmid # 194184 ; http://n2t.net/addgene:194184 ; RRID:Addgene_194184)

  • For your References section:

    ZNF354C is a transcriptional repressor that inhibits endothelial angiogenic sprouting. Oo JA, Irmer B, Gunther S, Warwick T, Palfi K, Izquierdo Ponce J, Teichmann T, Pfluger-Muller B, Gilsbach R, Brandes RP, Leisegang MS. Sci Rep. 2020 Nov 5;10(1):19079. doi: 10.1038/s41598-020-76193-0. 10.1038/s41598-020-76193-0 PubMed 33154469
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