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Addgene

pCMV6-MPP8-10xHis
(Plasmid #194178)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194178 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MPHOSPH8
  • Alt name
    M-Phase Phosphoprotein, Mpp8
  • Species
    H. sapiens (human)
  • Entrez Gene
    MPHOSPH8 (a.k.a. HSMPP8, TWA3, mpp8)
  • Promoter CMV
  • Tag / Fusion Protein
    • 10xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6-MPP8-10xHis was a gift from Ralf Brandes & Matthias Leisegang (Addgene plasmid # 194178 ; http://n2t.net/addgene:194178 ; RRID:Addgene_194178)

  • For your References section:

    HIF1alpha-AS1 is a DNA:DNA:RNA triplex-forming lncRNA interacting with the HUSH complex. Leisegang MS, Bains JK, Seredinski S, Oo JA, Krause NM, Kuo CC, Gunther S, Senturk Cetin N, Warwick T, Cao C, Boos F, Izquierdo Ponce J, Haydar S, Bednarz R, Valasarajan C, Fuhrmann DC, Preussner J, Looso M, Pullamsetti SS, Schulz MH, Jonker HRA, Richter C, Rezende F, Gilsbach R, Pfluger-Muller B, Wittig I, Grummt I, Ribarska T, Costa IG, Schwalbe H, Brandes RP. Nat Commun. 2022 Nov 2;13(1):6563. doi: 10.1038/s41467-022-34252-2. 10.1038/s41467-022-34252-2 PubMed 36323673
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