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pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)
(Plasmid #194175)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIF1a-AS1
  • Alt name
    5'AHIF1alpha
  • Alt name
    HIF1A Antisense RNA 1
  • Species
    H. sapiens (human)
  • Mutation
    Within Exon1, nucleotides 26-78 were removed
  • Entrez Gene
    HIF1A-AS1 (a.k.a. 5'aHIF-1A, 5'aHIF1alpha)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78) was a gift from Ralf Brandes & Matthias Leisegang (Addgene plasmid # 194175 ; http://n2t.net/addgene:194175 ; RRID:Addgene_194175)

  • For your References section:

    HIF1alpha-AS1 is a DNA:DNA:RNA triplex-forming lncRNA interacting with the HUSH complex. Leisegang MS, Bains JK, Seredinski S, Oo JA, Krause NM, Kuo CC, Gunther S, Senturk Cetin N, Warwick T, Cao C, Boos F, Izquierdo Ponce J, Haydar S, Bednarz R, Valasarajan C, Fuhrmann DC, Preussner J, Looso M, Pullamsetti SS, Schulz MH, Jonker HRA, Richter C, Rezende F, Gilsbach R, Pfluger-Muller B, Wittig I, Grummt I, Ribarska T, Costa IG, Schwalbe H, Brandes RP. Nat Commun. 2022 Nov 2;13(1):6563. doi: 10.1038/s41467-022-34252-2. 10.1038/s41467-022-34252-2 PubMed 36323673
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