pcDNA3.1_Tau Authentic Alu 10-12V337M
(Plasmid #194169)

• Purpose: Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.
• Depositing Lab: Stefan Stamm
• Publication: Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):12979-12996. doi: 10.1093/nar/gkac1129.

Backbone

• Vector backbone: pcDNA3.1
• Total vector size (bp): 8048
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Microtubule-associated protein tau 10-12 V337M
• Alt name: MAPT 10-12VM
• Species: H. sapiens (human)
• Insert Size (bp): 2640
• Mutation: Changed Valine 337 to Methinonine
• Tag / Fusion Protein:
  • 3X flag

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry