pcDNA3.1_Tau Authentic Alu 10-12K317M
(Plasmid
#194168)
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PurposeInsert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 8048
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMicrotubule-associated protein tau 10-12 K317M
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Alt nameMAPT 10-12 KM
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SpeciesH. sapiens (human)
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Insert Size (bp)2640
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MutationChanged Lysine 317 to Methionine
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Tag
/ Fusion Protein
- 3X Flag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_Tau Authentic Alu 10-12K317M was a gift from Stefan Stamm (Addgene plasmid # 194168 ; http://n2t.net/addgene:194168 ; RRID:Addgene_194168) -
For your References section:
RNA editing of microtubule-associated protein tau circular RNAs promotes their translation and tau tangle formation. Welden JR, Margvelani G, Arizaca Maquera KA, Gudlavalleti B, Miranda Sardon SC, Campos AR, Robil N, Lee DC, Hernandez AG, Wang WX, Di J, de la Grange P, Nelson PT, Stamm S. Nucleic Acids Res. 2022 Dec 9;50(22):12979-12996. doi: 10.1093/nar/gkac1129. 10.1093/nar/gkac1129 PubMed 36533443