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pcDNA3.1_ZKSCAN1_Tau10-12K317M
(Plasmid #194164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 7005
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Microtubule-associated protein tau 10-12 WT
  • Alt name
    MAPT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1650
  • Mutation
    Changed Lysine 317 to Methionine
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
  • Tag / Fusion Protein
    • 3X Flag

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_ZKSCAN1_Tau10-12K317M was a gift from Stefan Stamm (Addgene plasmid # 194164 ; http://n2t.net/addgene:194164 ; RRID:Addgene_194164)

  • For your References section:

    RNA editing of microtubule-associated protein tau circular RNAs promotes their translation and tau tangle formation. Welden JR, Margvelani G, Arizaca Maquera KA, Gudlavalleti B, Miranda Sardon SC, Campos AR, Robil N, Lee DC, Hernandez AG, Wang WX, Di J, de la Grange P, Nelson PT, Stamm S. Nucleic Acids Res. 2022 Dec 9;50(22):12979-12996. doi: 10.1093/nar/gkac1129. 10.1093/nar/gkac1129 PubMed 36533443
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