pcDNA3.1_ZKSCAN1_Tau10-12WT
(Plasmid
#194163)
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PurposeInsert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194163 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 7005
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMicrotubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
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SpeciesH. sapiens (human)
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Insert Size (bp)1650
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Tag
/ Fusion Protein
- 3X flag tag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_ZKSCAN1_Tau10-12WT was a gift from Stefan Stamm (Addgene plasmid # 194163 ; http://n2t.net/addgene:194163 ; RRID:Addgene_194163) -
For your References section:
RNA editing of microtubule-associated protein tau circular RNAs promotes their translation and tau tangle formation. Welden JR, Margvelani G, Arizaca Maquera KA, Gudlavalleti B, Miranda Sardon SC, Campos AR, Robil N, Lee DC, Hernandez AG, Wang WX, Di J, de la Grange P, Nelson PT, Stamm S. Nucleic Acids Res. 2022 Dec 9;50(22):12979-12996. doi: 10.1093/nar/gkac1129. 10.1093/nar/gkac1129 PubMed 36533443
