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pcDNA3-HA-Uch37C88A
(Plasmid #19416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19416 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Uch37
  • Alt name
    Uchl5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    C88A
  • GenBank ID
    Uchl5
  • Entrez Gene
    UCHL5 (a.k.a. CGI-70, INO80R, UCH-L5, UCH37)
  • Tag / Fusion Protein
    • 2XHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use translation from NM_015984, which corresponds to NP_057068, to determine protein sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-Uch37C88A was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 19416 ; http://n2t.net/addgene:19416 ; RRID:Addgene_19416)

  • For your References section:

    Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Yao T, Song L, Xu W, DeMartino GN, Florens L, Swanson SK, Washburn MP, Conaway RC, Conaway JW, Cohen RE. Nat Cell Biol. 2006 Sep . 8(9):994-1002. 10.1038/ncb1460 PubMed 16906146
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