pAd MafA-I-nGFP
(Plasmid #19412)

• Depositing Lab: Douglas Melton
• Publication: Zhou et al Nature. 2008 Aug 27. ():.

Backbone

• Vector backbone: pAd CMV/V5 DEST
• Backbone size w/o insert (bp): 34864
• Vector type: Adenoviral ; Entry Vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: Transform into TOP10 cells
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
• Alt name: Mafa
• Species: M. musculus (mouse)
• Insert Size (bp): 2660
• Entrez Gene: Mafa (a.k.a. RIPE3b1)
• Tags / Fusion Proteins:
  • IRES (C terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: Gateway (unknown if destroyed)
• 3′ cloning site: Gateway (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Qiuo Zhon
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene was unable to acquire sequence for this plasmid but is distributing the plasmid at the Melton Lab's request. Please address any problems with this plasmid to the Melton lab.