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His SUMO TEV R221C SPOP 28-359
(Plasmid #194116)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194116 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEXP
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPOP R221C
  • Species
    H. sapiens (human)
  • Mutation
    R221C
  • GenBank ID
    XM_024450995
  • Entrez Gene
    SPOP (a.k.a. BTBD32, NEDMACE, NEDMIDF, NSDVS1, NSDVS2, TEF2)
  • Tag / Fusion Protein
    • 6His-SUMO-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AGATTCTTGTACGACGGTATTAG
  • 3′ sequencing primer TAGTTATTGCTCAGCGGTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His SUMO TEV R221C SPOP 28-359 was a gift from Tanja Mittag (Addgene plasmid # 194116 ; http://n2t.net/addgene:194116 ; RRID:Addgene_194116)

  • For your References section:

    Conformational and oligomeric states of SPOP from small-angle X-ray scattering and molecular dynamics simulations. Thomasen FE, Cuneo MJ, Mittag T, Lindorff-Larsen K. Elife. 2023 Mar 1;12:e84147. doi: 10.7554/eLife.84147. 10.7554/eLife.84147 PubMed 36856266
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