pAd 2B Ngn3-I-nGFP
(Plasmid #19410)

• Depositing Lab: Douglas Melton
• Publication: Zhou et al Nature. 2008 Aug 27. ():.

Backbone

• Vector backbone: pAd CMV/V5 DEST
• Backbone size w/o insert (bp): 34864
• Vector type: Adenoviral ; Entry Vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: Transform into TOP10 cells
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: neurogenin 3
• Alt name: Neurog3, Ngn3, Atoh5; Math4B
• Species: M. musculus (mouse)
• Insert Size (bp): 2100
• Entrez Gene: Neurog3 (a.k.a. Atoh5, Math4B, bHLHa7, ngn3)
• Tags / Fusion Proteins:
  • IRES (C terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: Gateway (unknown if destroyed)
• 3′ cloning site: Gateway (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Qiuo Zhon
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry