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Addgene

nNc-ABE
(Plasmid #194095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 7359
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TadA 8e-nNcCas9
  • Species
    Synthetic
  • Mutation
    NcCas9 D16A
  • Promoter CMV

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer tagaaggcacagtcgaggctgat
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nNc-ABE was a gift from Zhanjun Li (Addgene plasmid # 194095 ; http://n2t.net/addgene:194095 ; RRID:Addgene_194095)

  • For your References section:

    Versatile and efficient genome editing with Neisseria cinerea Cas9. Liu Z, Chen S, Xie W, Yu H, Lai L, Li Z. Commun Biol. 2022 Nov 26;5(1):1296. doi: 10.1038/s42003-022-04258-z. 10.1038/s42003-022-04258-z PubMed 36435853
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