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Addgene

NcCas9
(Plasmid #194093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 3300
  • Total vector size (bp) 6765
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NcCas9
  • Species
    Synthetic
  • Insert Size (bp)
    3300
  • Promoter CMV

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer tagaaggcacagtcgaggctgat
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    NcCas9 synthesized by Genscript Biotech.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NcCas9 was a gift from Zhanjun Li (Addgene plasmid # 194093 ; http://n2t.net/addgene:194093 ; RRID:Addgene_194093)

  • For your References section:

    Versatile and efficient genome editing with Neisseria cinerea Cas9. Liu Z, Chen S, Xie W, Yu H, Lai L, Li Z. Commun Biol. 2022 Nov 26;5(1):1296. doi: 10.1038/s42003-022-04258-z. 10.1038/s42003-022-04258-z PubMed 36435853
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