Skip to main content
Addgene

piggyBAC-H2B FR-MQV
(Plasmid #194079)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    piggyBAC-H2B
  • Backbone manufacturer
    SBI
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FR-MQV
  • Alt name
    FusionRed M42Q, C159V, L175M
  • Species
    Synthetic; Entacmaea quadricolor
  • Insert Size (bp)
    699
  • Mutation
    Methionine 42 to Glutamine, Cystiene 159 to Valine, Leucine 175 to Methionine
  • Promoter CMV7
  • Tag / Fusion Protein
    • Histone H2B (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (introduced by PCR) (not destroyed)
  • 5′ sequencing primer H2B Forward primer (CATCACCAAGTACACCAGC)
  • 3′ sequencing primer FR internal reverse sequencing primer
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piggyBAC-H2B FR-MQV was a gift from Ralph Jimenez (Addgene plasmid # 194079 ; http://n2t.net/addgene:194079 ; RRID:Addgene_194079)

  • For your References section:

    Engineering of a Brighter Variant of the FusionRed Fluorescent Protein Using Lifetime Flow Cytometry and Structure-Guided Mutations. Mukherjee S, Hung ST, Douglas N, Manna P, Thomas C, Ekrem A, Palmer AE, Jimenez R. Biochemistry. 2020 Oct 6;59(39):3669-3682. doi: 10.1021/acs.biochem.0c00484. Epub 2020 Sep 24. 10.1021/acs.biochem.0c00484 PubMed 32914619
Related items:
Commonly requested with: