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Addgene

p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR
(Plasmid #194063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2T-CMV-BlastR
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    evoCjCas9-PEmax_dRnH
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR was a gift from Gerald Schwank (Addgene plasmid # 194063 ; http://n2t.net/addgene:194063 ; RRID:Addgene_194063)

  • For your References section:

    Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility. Schmidheini L, Mathis N, Marquart KF, Rothgangl T, Kissling L, Bock D, Chanez C, Wang JP, Jinek M, Schwank G. Nat Chem Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x. 10.1038/s41589-023-01427-x PubMed 37735239
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