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Addgene

pGWT35S-mCherry-NLS
(Plasmid #194049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGWT35S
  • Total vector size (bp) 4327
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    714
  • Promoter CaMV 35S promoter
  • Tag / Fusion Protein
    • SV40 (Nuclear localization signal) (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGTGAGCAAGGGCGAGGAGGA
  • 3′ sequencing primer GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACACCTTGCGCTTCTTCTTAGGTCCCGACTTGTACAGCTCGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWT35S-mCherry-NLS was a gift from Yutaka Kodama (Addgene plasmid # 194049 ; http://n2t.net/addgene:194049 ; RRID:Addgene_194049)

  • For your References section:

    Organellar Glue: A Molecular Tool to Artificially Control Chloroplast-Chloroplast Interactions. Ichikawa S, Kato S, Fujii Y, Ishikawa K, Numata K, Kodama Y. ACS Synth Biol. 2022 Oct 21;11(10):3190-3197. doi: 10.1021/acssynbio.2c00367. Epub 2022 Sep 30. 10.1021/acssynbio.2c00367 PubMed 36178266
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