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Addgene

pCM3.35
(Plasmid #19404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR201
  • Backbone size w/o insert (bp) 2257
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    par-5 ORF+3' in pDONR201
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1909
  • GenBank ID
    NM_069834
  • Entrez Gene
    par-5 (a.k.a. CELE_M117.2)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP1 (destroyed during cloning)
  • 3′ cloning site attP2 (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM3.35 was a gift from Geraldine Seydoux (Addgene plasmid # 19404 ; http://n2t.net/addgene:19404 ; RRID:Addgene_19404)

  • For your References section:

    3' UTRs Are the Primary Regulators of Gene Expression in the C. elegans Germline. Merritt C, Rasoloson D, Ko D, Seydoux G. Curr Biol. 2008 Sep 23. ():. 10.1016/j.cub.2008.08.013 PubMed 18818082
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