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RcSxph E540D
(Plasmid #194020)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rana catesbeiana Saxiphilin
  • Species
    Rana catesbeiana
  • Mutation
    E540D
  • GenBank ID
    U05246.1
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • mEGFP, (C terminal on insert)
    • His10 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer Polyhedrin forward
  • 3′ sequencing primer GFP reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation numbering scheme is based on the predicted signal peptide (SP) cleavage site and does not include SP.

Relative to U05246.1, RcSxph contains an additional M following signal peptide.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RcSxph E540D was a gift from Dan Minor (Addgene plasmid # 194020 ; http://n2t.net/addgene:194020 ; RRID:Addgene_194020)

  • For your References section:

    Definition of a saxitoxin (STX) binding code enables discovery and characterization of the anuran saxiphilin family. Chen Z, Zakrzewska S, Hajare HS, Alvarez-Buylla A, Abderemane-Ali F, Bogan M, Ramirez D, O'Connell LA, Du Bois J, Minor DL Jr. Proc Natl Acad Sci U S A. 2022 Nov;119(44):e2210114119. doi: 10.1073/pnas.2210114119. Epub 2022 Oct 24. 10.1073/pnas.2210114119 PubMed 36279441
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