RcSxph
(Plasmid
#194018)
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PurposeProtein expression and characterization
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 194018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFastBac1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRana catesbeiana Saxiphilin
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SpeciesRana catesbeiana
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GenBank IDU05246.1
- Promoter Polyhedrin
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Tags
/ Fusion Proteins
- mEGFP, (C terminal on insert)
- His10 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Polyhedrin forward
- 3′ sequencing primer GFP reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Relative to U05246.1, RcSxph contains extra M following signal peptide.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RcSxph was a gift from Dan Minor (Addgene plasmid # 194018 ; http://n2t.net/addgene:194018 ; RRID:Addgene_194018) -
For your References section:
Definition of a saxitoxin (STX) binding code enables discovery and characterization of the anuran saxiphilin family. Chen Z, Zakrzewska S, Hajare HS, Alvarez-Buylla A, Abderemane-Ali F, Bogan M, Ramirez D, O'Connell LA, Du Bois J, Minor DL Jr. Proc Natl Acad Sci U S A. 2022 Nov;119(44):e2210114119. doi: 10.1073/pnas.2210114119. Epub 2022 Oct 24. 10.1073/pnas.2210114119 PubMed 36279441