Skip to main content
Addgene

pIS003
(Plasmid #194014)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194014 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pYTK084
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C16 Ex4 XTR f1
  • Species
    M. musculus (mouse)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.11.21.517425 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIS003 was a gift from Timothy Whitehead (Addgene plasmid # 194014 ; http://n2t.net/addgene:194014 ; RRID:Addgene_194014)

  • For your References section:

    A method for generating user-defined circular single-stranded DNA from plasmid DNA using Golden Gate intramolecular ligation. Strawn IK, Steiner PJ, Newton MS, Baumer ZT, Whitehead TA. Biotechnol Bioeng. 2023 Oct;120(10):3057-3066. doi: 10.1002/bit.28471. Epub 2023 Jun 27. 10.1002/bit.28471 PubMed 37366288
Related items: