pIS001
(Plasmid
#194012)
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PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYTK084
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameROSA-TRE-C16V5-G-pA
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.11.21.517425 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIS001 was a gift from Timothy Whitehead (Addgene plasmid # 194012 ; http://n2t.net/addgene:194012 ; RRID:Addgene_194012) -
For your References section:
A method for generating user-defined circular single-stranded DNA from plasmid DNA using Golden Gate intramolecular ligation. Strawn IK, Steiner PJ, Newton MS, Baumer ZT, Whitehead TA. Biotechnol Bioeng. 2023 Oct;120(10):3057-3066. doi: 10.1002/bit.28471. Epub 2023 Jun 27. 10.1002/bit.28471 PubMed 37366288
