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mCh-Miro1(∆TM)-SspB
(Plasmid #194007)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194007 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 7000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Miro1 (var4, peroxisomal splice variant)
  • Alt name
    Miro1 var4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2076
  • GenBank ID
    NM_001033568.3
  • Entrez Gene
    RHOT1 (a.k.a. ARHT1, MIRO-1, MIRO1)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCh-Miro1(∆TM)-SspB was a gift from Pietro De Camilli (Addgene plasmid # 194007 ; http://n2t.net/addgene:194007 ; RRID:Addgene_194007)

  • For your References section:

    VPS13D bridges the ER to mitochondria and peroxisomes via Miro. Guillen-Samander A, Leonzino M, Hanna MG, Tang N, Shen H, De Camilli P. J Cell Biol. 2021 May 3;220(5). pii: 212021. doi: 10.1083/jcb.202010004. 10.1083/jcb.202010004 PubMed 33891013
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