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Addgene

pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA
(Plasmid #193968)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193968 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPol eta
  • Species
    H. sapiens (human)
  • Entrez Gene
    POLH (a.k.a. RAD30, RAD30A, XP-V, XPV)
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA was a gift from Huawei Tong (Addgene plasmid # 193968 ; http://n2t.net/addgene:193968 ; RRID:Addgene_193968)
  • For your References section:

    Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase. Tong H, Wang X, Liu Y, Liu N, Li Y, Luo J, Ma Q, Wu D, Li J, Xu C, Yang H. Nat Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01595-6. 10.1038/s41587-022-01595-6 PubMed 36624150