pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA
(Plasmid
#193968)
-
PurposeEncodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 193968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCAG
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehPol eta
-
SpeciesH. sapiens (human)
-
Entrez GenePOLH (a.k.a. RAD30, RAD30A, XP-V, XPV)
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA was a gift from Huawei Tong (Addgene plasmid # 193968 ; http://n2t.net/addgene:193968 ; RRID:Addgene_193968) -
For your References section:
Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase. Tong H, Wang X, Liu Y, Liu N, Li Y, Luo J, Ma Q, Wu D, Li J, Xu C, Yang H. Nat Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01595-6. 10.1038/s41587-022-01595-6 PubMed 36624150