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PurposeAn engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193967 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF1a
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameecTadA8e-nSpCas9(D10A)-MPG(v3)
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SpeciesSynthetic
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MutationG163R, N169S, S198A, K202A, G203A, S206A, K210A
- Promoter hU6, EF1a, CBh
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AYBEv3 was a gift from Huawei Tong (Addgene plasmid # 193967 ; http://n2t.net/addgene:193967 ; RRID:Addgene_193967) -
For your References section:
Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase. Tong H, Wang X, Liu Y, Liu N, Li Y, Luo J, Ma Q, Wu D, Li J, Xu C, Yang H. Nat Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01595-6. 10.1038/s41587-022-01595-6 PubMed 36624150
