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Addgene

AYBEv0.1
(Plasmid #193966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1a
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ecTadA8e-nSpCas9(D10A)-MPG(wt)
  • Species
    Synthetic
  • Promoter hU6, EF1a, CBh

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AYBEv0.1 was a gift from Huawei Tong (Addgene plasmid # 193966 ; http://n2t.net/addgene:193966 ; RRID:Addgene_193966)

  • For your References section:

    Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase. Tong H, Wang X, Liu Y, Liu N, Li Y, Luo J, Ma Q, Wu D, Li J, Xu C, Yang H. Nat Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01595-6. 10.1038/s41587-022-01595-6 PubMed 36624150
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