pCEV-G2-Km ymScarletI
(Plasmid
#193960)
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PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCEV-G2-KM
- Backbone size w/o insert (bp) 6800
- Total vector size (bp) 7600
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Vector typeYeast Expression
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Selectable markersNeomycin (select with G418) ; G418
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameymScarletI
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SpeciesSynthetic
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Insert Size (bp)800
- Promoter TEF1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (unknown if destroyed)
- 3′ cloning site SacII (unknown if destroyed)
- 5′ sequencing primer taatacgactcactatagg
- 3′ sequencing primer aaatagggacctagacttc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCEV-G2-Km ymScarletI was a gift from Bas Teusink (Addgene plasmid # 193960 ; http://n2t.net/addgene:193960 ; RRID:Addgene_193960)