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Addgene

pCEV-G2-Km ymNeongreen
(Plasmid #193959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEV-G2-KM
  • Backbone size w/o insert (bp) 700
  • Total vector size (bp) 7800
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418) ; G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ymNeongreen
  • Species
    Synthetic
  • Insert Size (bp)
    800
  • Promoter TEF1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (unknown if destroyed)
  • 3′ cloning site SacII (unknown if destroyed)
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer aaatagggacctagacttc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEV-G2-Km ymNeongreen was a gift from Bas Teusink (Addgene plasmid # 193959 ; http://n2t.net/addgene:193959 ; RRID:Addgene_193959)
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