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pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1
(Plasmid #193942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSGV-1D3-28Z All ITAMs intact
  • Backbone manufacturer
    Steven Rosenberg
  • Backbone size w/o insert (bp) 7013
  • Total vector size (bp) 7547
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
  • Species
    Synthetic
  • Insert Size (bp)
    719

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TACACAGTCCTGCTGACCA
  • 3′ sequencing primer cgtaacagaacagcacgc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1 was a gift from Greg Delgoffe (Addgene plasmid # 193942 ; http://n2t.net/addgene:193942 ; RRID:Addgene_193942)

  • For your References section:

    Fully murine CD105-targeted CAR-T cells provide an immunocompetent model for CAR-T cell biology. Lontos K, Wang Y, Colbert M, Kumar A, Joshi S, Philbin M, Wang Y, Frisch A, Lohmueller J, Rivadeneira DB, Delgoffe GM. Oncoimmunology. 2022 Oct 18;11(1):2131229. doi: 10.1080/2162402X.2022.2131229. eCollection 2022. 10.1080/2162402X.2022.2131229 PubMed 36275862
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