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Addgene

pBa.Halo-KIF3B DD 593-747
(Plasmid #193933)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193933 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBeta Actin-HALO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kif 3B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    471
  • Entrez Gene
    Kif3b (a.k.a. AI854312, AW549267, mKIAA0359)
  • Promoter Chicken Beta actin
  • Tag / Fusion Protein
    • Halo (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBa.Halo-KIF3B DD 593-747 was a gift from Marvin Bentley (Addgene plasmid # 193933 ; http://n2t.net/addgene:193933 ; RRID:Addgene_193933)
  • For your References section:

    KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC. Garbouchian A, Montgomery A, Gilbert SP, Bentley M. Mol Biol Cell. 2022 Oct 6:mbcE22080336. doi: 10.1091/mbc.E22-08-0336. 10.1091/mbc.E22-08-0336 PubMed 36200888