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pET21a HsDHFR-bio-10His
(Plasmid #193883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6093
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human dihydrofolate reductase (DHFR)
  • Alt name
    DYR; DHFR1; DHFRP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    669
  • Mutation
    Codon optimized
  • GenBank ID
    1719
  • Entrez Gene
    DHFR (a.k.a. DHFR1, DHFRP1, DYR)
  • Promoter T7
  • Tags / Fusion Proteins
    • biotinylation sequence (C terminal on insert)
    • His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Hind3 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a HsDHFR-bio-10His was a gift from James Inglese (Addgene plasmid # 193883 ; http://n2t.net/addgene:193883 ; RRID:Addgene_193883)

  • For your References section:

    Methotrexate-based PROTACs as DHFR-specific chemical probes. Rana S, Dranchak P, Dahlin JL, Lamy L, Li W, Oliphant E, Shrimp JH, Rajacharya GH, Tharakan R, Holland DO, Whitten AS, Wilson KM, Singh PK, Durum SK, Tao D, Rai G, Inglese J. Cell Chem Biol. 2024 Feb 15;31(2):221-233.e14. doi: 10.1016/j.chembiol.2023.09.014. Epub 2023 Oct 23. 10.1016/j.chembiol.2023.09.014 PubMed 37875111
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