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pOPINB-HOIL-1 helix-RBR (233–510)
(Plasmid #193859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOPINB
  • Backbone size w/o insert (bp) 5642
  • Total vector size (bp) 6147
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HOIL-1
  • Alt name
    Heme-oxidized IRP2 ubiquitin ligase 1
  • Alt name
    RBCK1 C20orf18, HOIL-1, HOIL1, PBMEI, PGBM1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4
  • Alt name
    RanBP-type and C3HC4-type zinc finger-containing protein 1
  • Species
    H. sapiens (human)
  • Mutation
    Residues 233–510, codon optimised for expression in E. coli
  • GenBank ID
    NM_031229
  • Entrez Gene
    RBCK1 (a.k.a. C20orf18, HOIL-1, HOIL1, PBMEI, PGBM1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6x-His tag (N terminal on backbone)
    • 3C protease cleavage site (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Helix-RBR construct suitable for in vitro activity assays and crystallisation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINB-HOIL-1 helix-RBR (233–510) was a gift from Bernhard Lechtenberg (Addgene plasmid # 193859 ; http://n2t.net/addgene:193859 ; RRID:Addgene_193859)

  • For your References section:

    The unifying catalytic mechanism of the RING-between-RING E3 ubiquitin ligase family. Wang XS, Cotton TR, Trevelyan SJ, Richardson LW, Lee WT, Silke J, Lechtenberg BC. Nat Commun. 2023 Jan 11;14(1):168. doi: 10.1038/s41467-023-35871-z. 10.1038/s41467-023-35871-z PubMed 36631489
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