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Addgene

Sniper2L
(Plasmid #193856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUGW
  • Total vector size (bp) 12859
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sniper2L
  • Alt name
    Sniper2L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4641
  • Mutation
    E1007L
  • Promoter EFS
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • FLAG (C terminal on insert)
    • BSD (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer 5' - ggtcttgaaaggagtgggaattgg - 3'
  • 3′ sequencing primer 5' - CAGGTCGCTTGTCGCCTCC - 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sniper2L was a gift from Hyongbum Kim (Addgene plasmid # 193856 ; http://n2t.net/addgene:193856 ; RRID:Addgene_193856)

  • For your References section:

    Sniper2L is a high-fidelity Cas9 variant with high activity. Kim YH, Kim N, Okafor I, Choi S, Min S, Lee J, Bae SM, Choi K, Choi J, Harihar V, Kim Y, Kim JS, Kleinstiver BP, Lee JK, Ha T, Kim HH. Nat Chem Biol. 2023 Mar 9. doi: 10.1038/s41589-023-01279-5. 10.1038/s41589-023-01279-5 PubMed 36894722
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