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Addgene

SpCas9 TadDE
(Plasmid #193837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193837 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV with a BR322 origin
  • Total vector size (bp) 8780
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TadA-DualEditor-Cas9D10A-2xUGI
  • Alt name
    TadDE
  • Insert Size (bp)
    5380

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpCas9 TadDE was a gift from David Liu (Addgene plasmid # 193837 ; http://n2t.net/addgene:193837 ; RRID:Addgene_193837)

  • For your References section:

    Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity. Neugebauer ME, Hsu A, Arbab M, Krasnow NA, McElroy AN, Pandey S, Doman JL, Huang TP, Raguram A, Banskota S, Newby GA, Tolar J, Osborn MJ, Liu DR. Nat Biotechnol. 2022 Nov 10. pii: 10.1038/s41587-022-01533-6. doi: 10.1038/s41587-022-01533-6. 10.1038/s41587-022-01533-6 PubMed 36357719
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