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pET29b-10xHis-Super TEV
(Plasmid #193833)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193833 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TEV protease
  • Species
    Tobacco etch virus
  • Mutation
    T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180A, S219V
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutations taken from:
Wei et al., 2012. Protein Expression and Purification
Sanchez and Ting, 2019. Nature Methods
van den Berg et al., 2006. Journal of Biotechnology
Cabrita et al., 2007. Protein Science
Kapust et al., 2002. Biochemical and Biophysical Research Communications

The protocol for the purification of 10xHis-SuperTEV is available on protocols.io: dx.doi.org/10.17504/protocols.io.yxmvm2zxng3p/v1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-10xHis-Super TEV was a gift from EPFL Protein Production and Structure Core Facility (PTPSP) & Florence Pojer (Addgene plasmid # 193833 ; http://n2t.net/addgene:193833 ; RRID:Addgene_193833)

  • For your References section:

    Purification of 10xHis-SuperTEV. Lau K, Bouchri B, Pojer F. protocols.io, doi: 10.17504/protocols.io.yxmvm2zxng3p/v1 10.17504/protocols.io.yxmvm2zxng3p/v1
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