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Addgene

pCM5.50
(Plasmid #19383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONRP2RP3
  • Backbone size w/o insert (bp) 2641
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spn-4 3'utr in pDONRP2RP3
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    502
  • GenBank ID
    NM_072316
  • Entrez Gene
    spn-4 (a.k.a. CELE_ZC404.8)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP2R (destroyed during cloning)
  • 3′ cloning site attP3 (destroyed during cloning)
  • 5′ sequencing primer M13Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM5.50 was a gift from Geraldine Seydoux (Addgene plasmid # 19383 ; http://n2t.net/addgene:19383 ; RRID:Addgene_19383)

  • For your References section:

    3' UTRs Are the Primary Regulators of Gene Expression in the C. elegans Germline. Merritt C, Rasoloson D, Ko D, Seydoux G. Curr Biol. 2008 Sep 23. ():. 10.1016/j.cub.2008.08.013 PubMed 18818082
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