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Addgene

pCDNA5/FRT-ChemoB
(Plasmid #193808)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193808 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ChemoB
  • Species
    Synthetic
  • Insert Size (bp)
    1600
  • Mutation
    EBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L271E
  • Promoter CMV
  • Tag / Fusion Protein
    • 10xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA5/FRT-ChemoB was a gift from Julien Hiblot (Addgene plasmid # 193808 ; http://n2t.net/addgene:193808 ; RRID:Addgene_193808)

  • For your References section:

    A general method for the development of multicolor biosensors with large dynamic ranges. Hellweg L, Edenhofer A, Barck L, Huppertz MC, Frei MS, Tarnawski M, Bergner A, Koch B, Johnsson K, Hiblot J. Nat Chem Biol. 2023 Jun 8. doi: 10.1038/s41589-023-01350-1. 10.1038/s41589-023-01350-1 PubMed 37291200
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