pCM5.56
(Plasmid
#19379)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19379 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONRP2RP3
- Backbone size w/o insert (bp) 2641
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemsp-142 3'utr in pDONRP2RP3
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SpeciesC. elegans (nematode)
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Insert Size (bp)64
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attP2R (destroyed during cloning)
- 3′ cloning site attP3 (destroyed during cloning)
- 5′ sequencing primer M13Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM5.56 was a gift from Geraldine Seydoux (Addgene plasmid # 19379 ; http://n2t.net/addgene:19379 ; RRID:Addgene_19379) -
For your References section:
3' UTRs Are the Primary Regulators of Gene Expression in the C. elegans Germline. Merritt C, Rasoloson D, Ko D, Seydoux G. Curr Biol. 2008 Sep 23. ():. 10.1016/j.cub.2008.08.013 PubMed 18818082