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Addgene

pDA2ScIfm
(Plasmid #193786)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193786 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZ
  • Backbone size w/o insert (bp) 2861
  • Total vector size (bp) 3574
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Frame-shifted CI
  • Species
    Mutated Lambda CI
  • Insert Size (bp)
    713
  • Promoter PLTATAA synthetic promoter carrying binding sites for LacI and TetR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer AAAGAAACCATTA ACACAAGAGCA
  • 3′ sequencing primer TCAGCCAAACGTC TCTTCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDA2ScIfm was a gift from Sangram Bagh (Addgene plasmid # 193786 ; http://n2t.net/addgene:193786 ; RRID:Addgene_193786)

  • For your References section:

    A frame-shifted gene, which rescued its function by non-natural start codons and its application in constructing synthetic gene circuits. Sarkar K, Mukhopadhyay S, Bonnerjee D, Srivastava R, Bagh S. J Biol Eng. 2019 Mar 1;13:20. doi: 10.1186/s13036-019-0151-x. eCollection 2019. 10.1186/s13036-019-0151-x PubMed 30867677
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