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Addgene

pGL4.0-TERT_-100_-79
(Plasmid #193785)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193785 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL4.0
  • Backbone manufacturer
    Promega
  • Total vector size (bp) 4418
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TERT promoter
  • Alt name
    telomerase reverse transcriptase
  • Species
    H. sapiens (human)
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
  • Promoter TERT

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.0-TERT_-100_-79 was a gift from Joseph Costello (Addgene plasmid # 193785 ; http://n2t.net/addgene:193785 ; RRID:Addgene_193785)
  • For your References section:

    Conserved features of TERT promoter duplications reveal an activation mechanism that mimics hotspot mutations in cancer. Barger CJ, Suwala AK, Soczek KM, Wang AS, Kim MY, Hong C, Doudna JA, Chang SM, Phillips JJ, Solomon DA, Costello JF. Nat Commun. 2022 Sep 16;13(1):5430. doi: 10.1038/s41467-022-33099-x. 10.1038/s41467-022-33099-x PubMed 36114166