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Addgene

pRA1SEGFPTcIfm
(Plasmid #193777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZ
  • Backbone size w/o insert (bp) 2367
  • Total vector size (bp) 4479
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
  • Species
    Bacteria
  • Insert Size (bp)
    2112
  • Promoter PR promoter and PLTetO-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (unknown if destroyed)
  • 3′ cloning site AvrII (unknown if destroyed)
  • 5′ sequencing primer GTTTTGGAGCACG GAAAGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRA1SEGFPTcIfm was a gift from Sangram Bagh (Addgene plasmid # 193777 ; http://n2t.net/addgene:193777 ; RRID:Addgene_193777)

  • For your References section:

    A frame-shifted gene, which rescued its function by non-natural start codons and its application in constructing synthetic gene circuits. Sarkar K, Mukhopadhyay S, Bonnerjee D, Srivastava R, Bagh S. J Biol Eng. 2019 Mar 1;13:20. doi: 10.1186/s13036-019-0151-x. eCollection 2019. 10.1186/s13036-019-0151-x PubMed 30867677
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