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pC1-ND-Cdt1-mCitrine
(Plasmid #193771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pC1
  • Backbone size w/o insert (bp) 4827
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ND-Cdt1-mCitrine
  • Alt name
    Non-degradable Cdt1-mCitrine
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2387
  • Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 to alanine), SV40 NLS, 1-183 bp codon optimized
  • Entrez Gene
    CDT1 (a.k.a. DUP, RIS2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCitrine (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC1-ND-Cdt1-mCitrine was a gift from Tobias Meyer (Addgene plasmid # 193771 ; http://n2t.net/addgene:193771 ; RRID:Addgene_193771)

  • For your References section:

    CDT1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis. Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T. Mol Cell. 2023 Jan 5;83(1):26-42.e13. doi: 10.1016/j.molcel.2022.12.004. 10.1016/j.molcel.2022.12.004 PubMed 36608667
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