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pCW-ND-Cdt1ΔR198A/R210A-HA-Puro
(Plasmid #193769)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW
  • Backbone size w/o insert (bp) 7606
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ND-Cdt1ΔR198A/R210A
  • Alt name
    Non-degradable Cdt1ΔR198A/R210A-HA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1689
  • Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 to alanine), R198A, R210A, SV40 NLS, 1-183 bp codon optimized
  • Entrez Gene
    CDT1 (a.k.a. DUP, RIS2)
  • Promoter TRE
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cagagctcgtttagtgaaccg
  • 3′ sequencing primer cggaaccacgcccagagc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 3rd generation packaging system (pMDLg/pRRE, pRSV-rev, and pVSVG) was used to create virus from this lentiviral transfer vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW-ND-Cdt1ΔR198A/R210A-HA-Puro was a gift from Tobias Meyer (Addgene plasmid # 193769 ; http://n2t.net/addgene:193769 ; RRID:Addgene_193769)

  • For your References section:

    CDT1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis. Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T. Mol Cell. 2023 Jan 5;83(1):26-42.e13. doi: 10.1016/j.molcel.2022.12.004. 10.1016/j.molcel.2022.12.004 PubMed 36608667
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