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Addgene

pPANI2A2E2-Crimson
(Plasmid #193758)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193758 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZ
  • Backbone size w/o insert (bp) 2252
  • Total vector size (bp) 2930
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2-Crimson
  • Species
    Bacteria
  • Insert Size (bp)
    678
  • Promoter PANI2 synthetic promoter carrying binding sites for lambda CI and TetR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer TGGATAGCACTGAGAACGTCAT
  • 3′ sequencing primer ACCACGGTGTAGTCCTCGTT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPANI2A2E2-Crimson was a gift from Sangram Bagh (Addgene plasmid # 193758 ; http://n2t.net/addgene:193758 ; RRID:Addgene_193758)

  • For your References section:

    A single layer artificial neural network type architecture with molecular engineered bacteria for reversible and irreversible computing. Sarkar K, Bonnerjee D, Srivastava R, Bagh S. Chem Sci. 2021 Nov 9;12(48):15821-15832. doi: 10.1039/d1sc01505b. eCollection 2021 Dec 15. 10.1039/d1sc01505b PubMed 35024106
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