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Addgene

pO3
(Plasmid #193748)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193748 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZ
  • Backbone size w/o insert (bp) 2158
  • Total vector size (bp) 2836
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E2-Crimson
  • Species
    Bacteria
  • Insert Size (bp)
    678
  • Promoter PAAH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer TGGATAGCACTGAGAACGTCAT
  • 3′ sequencing primer ACCACGGTGTAGTCCTCGTT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pO3 was a gift from Sangram Bagh (Addgene plasmid # 193748 ; http://n2t.net/addgene:193748 ; RRID:Addgene_193748)

  • For your References section:

    Distributed Computing with Engineered Bacteria and Its Application in Solving Chemically Generated 2 x 2 Maze Problems. Sarkar K, Chakraborty S, Bonnerjee D, Bagh S. ACS Synth Biol. 2021 Oct 15;10(10):2456-2464. doi: 10.1021/acssynbio.1c00279. Epub 2021 Sep 20. 10.1021/acssynbio.1c00279 PubMed 34543017
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