pMPRAduo:minP:GFP
(Plasmid
#193739)
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PurposeMPRAduo ORF (minP:GFP) donor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.23
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Backbone manufacturerPromega
- Total vector size (bp) 3296
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFP
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SpeciesH. sapiens (human)
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Insert Size (bp)726
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.02.11.479757v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMPRAduo:minP:GFP was a gift from Ryan Tewhey (Addgene plasmid # 193739 ; http://n2t.net/addgene:193739 ; RRID:Addgene_193739) -
For your References section:
Whole-genome functional characterization of RE1 silencers using a modified massively parallel reporter assay. Mouri K, Dewey HB, Castro R, Berenzy D, Kales S, Tewhey R. Cell Genom. 2022 Dec 16;3(1):100234. doi: 10.1016/j.xgen.2022.100234. eCollection 2023 Jan 11. 10.1016/j.xgen.2022.100234 PubMed 36777181