pRAC10A
(Plasmid
#193734)
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PurposeExpresses deactivated-Cas9 (J23110) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 193734 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA331
- Total vector size (bp) 8624
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedCas9
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRAC10A was a gift from Martin Buck (Addgene plasmid # 193734 ; http://n2t.net/addgene:193734 ; RRID:Addgene_193734)