Skip to main content
Addgene

pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA
(Plasmid #193728)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene #20297)
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size w/o insert (bp) 5601
  • Total vector size (bp) 7842
  • Modifications to backbone
    Floxed ClopHensorN was generated by subcloning the inverted sequence of ClopHensorN (from Addgene plasmid # 50758) into the backbone of the double-floxed plasmid (Addgene plasmid # 20297), which had been prepared by removing the insert.
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ClopHensorN
  • Alt name
    E2GFP fused to Tandem Tomato
  • Species
    Synthetic
  • Insert Size (bp)
    2264
  • Promoter EF1a
  • Tag / Fusion Protein
    • Strep-II (N terminal on insert) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA was a gift from Colin Akerman (Addgene plasmid # 193728 ; http://n2t.net/addgene:193728 ; RRID:Addgene_193728)

  • For your References section:

    A genetically targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations. Calin A, Waseem T, Raimondo JV, Newey SE, Akerman CJ. iScience. 2023 Mar 9;26(4):106363. doi: 10.1016/j.isci.2023.106363. eCollection 2023 Apr 21. 10.1016/j.isci.2023.106363 PubMed 37034992
Related items: