LifeAct-mCherry
(Plasmid
#193723)
-
PurposeLabeling microtubules in living mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 193723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneB-actin
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameLifeAct-mCherry
-
SpeciesH. sapiens (human), Synthetic
-
MutationmCherry: Met1 Deleted
- Promoter Chicken beta-actin
-
Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
internal plasmid ID: pMB33/pMA192
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LifeAct-mCherry was a gift from Lukas Kapitein (Addgene plasmid # 193723 ; http://n2t.net/addgene:193723 ; RRID:Addgene_193723) -
For your References section:
Local changes in microtubule network mobility instruct neuronal polarization and axon specification. Burute M, Jansen KI, Mihajlovic M, Vermonden T, Kapitein LC. Sci Adv. 2022 Nov 4;8(44):eabo2343. doi: 10.1126/sciadv.abo2343. Epub 2022 Nov 4. 10.1126/sciadv.abo2343 PubMed 36332030