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pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB
(Plasmid #193719)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 193719 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    B-actin
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    hsKIF5B(aa1-560), RIGOR: G234A
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
  • Promoter Chicken beta-actin
  • Tag / Fusion Protein
    • 2xHA-FRB (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

internal plasmid ID: pKJ119

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB was a gift from Lukas Kapitein (Addgene plasmid # 193719 ; http://n2t.net/addgene:193719 ; RRID:Addgene_193719)

  • For your References section:

    Local changes in microtubule network mobility instruct neuronal polarization and axon specification. Burute M, Jansen KI, Mihajlovic M, Vermonden T, Kapitein LC. Sci Adv. 2022 Nov 4;8(44):eabo2343. doi: 10.1126/sciadv.abo2343. Epub 2022 Nov 4. 10.1126/sciadv.abo2343 PubMed 36332030
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